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 ที่มา:การประชุมวิชาการ ครั้งที่ 49 มหาวิทยาลัยเกษตรศาสตร์ (Proceeding of the 49th Annual Conference)หัวเรื่อง:คาริโอไทป์มาตรฐานและเครื่องหมายดีเอ็นเอบ่งชี้เพศปลานิล (Oreochromis aureus) |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Biochemical Properties of Nile Tilapia (Oreochromis niloticus) Hemoglobin) ผู้เขียน: เกรียงไกร ทองสาร,  ดร.วันชัย วรวัฒนเมธีกุล, รองศาสตราจารย์ , ดร.สุริยัน ธัญกิจจานุกิจ, ผู้ช่วยศาสตราจารย์ , นางชูศรี ศรีเพ็ญ, ผู้ช่วยศาสตราจารย์ , ดร.อาภัสสรา ชูเทศะ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractHemoglobins of O. niloticus (averaged standard length of 18.07 ? 0.89 cm and weight of 216.86 ? 27.13 g) were analysed of some biochemical properties. Average hematocrits were 25.80 ? 3.71% and average hemoglobin concentrations were 7.05 ? 1.08 g/dl. It was found that there were 9 – 11 types of hemoglobin by cellulose acetate electrophoresis. The anodic and cathodic hemoglobins were revealed by native polyacrylamide gel electrophoresis with approximate amounts of 45.72 and 54.28% of total hemoglobin, respectively. In addition, the cathodic hemoglobin was classified by cellulose acetate electrophoresis as cathodic I and cathodic II with approximate amounts of 47.62 and 6.66% of total hemoglobin, respectively. Mass spectrometry was performed to determine molecular weights of hemoglobin. It was found that the molecular weights of native hemoglobin was 63.0 kDa and the molecular weights of subunits were 15.3, 15.6 and 16.1 kDa. The pIs of anodic and cathodic hemoglobins ranged 5.28 – 6.80 and 7.27 – 9.71 , respectively. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characteristics of Collagen from Nile Tilapia (Oreochromis niloticus) Skin Isolated by Two Different Methods) ผู้เขียน:ตรีสินธุ์ โพธารส, ดร.นงนุช รักสกุลไทย, รองศาสตราจารย์, Jiraporn Runglerdkreangkrai, ดร.วันชัย วรวัฒนเมธีกุล, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractAcid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) isolated from Nile tilapia skin using two different methods were compared for biochemical properties and thermal stability. Method 1 (the Noitup method) was performed at 4-6?C for the whole process with a centrifuge speed of 30,000 g, while Method 2 (the Ogawa method) was performed at a higher temperature of 22-23?C, with a centrifuge speed of 10,000 g. The dry weight yields of ASC from Methods 1 and 2 were 38.84 and 20.70%, while the dry weight yields of PSC were 48.21 and 38.27%, respectively. The denaturation temperatures of ASC from Methods 1 and 2 were 34.29 and 34.43?C, while those of PSC were 34.32 and 34.61?C, respectively. From these amino acid and molecular weight profiles and SDS-PAGE results, ASC and PSC obtained by both methods were characterized as type I collagen with no disulfide bond, which were composed of ?-1 (2 chains) ?-2 (1 chain), ?, and ? subunits. Amino acid profiles of ASC and PSC obtained from both methods were similar. Except for the lower yield obtained from Method 2, there were no differences in the molecular weight, amino acid composition and denaturation temperature between ASC and PSC obtained from both methods. |
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ผลงานตีพิมพ์ในวารสารวิชาการCombining segments 9 and 10 in DNA and recombinant protein vaccines conferred superior protection against tilapia lake virus in hybrid red tilapia (oreochromis sp.) compared to single segment vaccinesผู้แต่ง:Chamtim, P., Dr.Eukote Suwan, Assistant Professor, Dong, H.T., Mr.Soranuth Sirisuay, Lecturer , Areechon, N., Wangkahart, E., Hirono, I., Mavichak, R., Dr.Sasimanas Unajak, Associate Professor , วารสาร: 
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